All of the stresses was derivatives from W303 (KWY165) to the following the exclusions: KWY7227 and you will KWY7246 was derivatives of BY4741 (KWY1601)

All of the stresses was derivatives from W303 (KWY165) to the following the exclusions: KWY7227 and you will KWY7246 was derivatives of BY4741 (KWY1601)

Yeast Strains and you can Growth Criteria

All strains are listed in Supplementary file 2. gcn2?, CDC33-IAA7-3V5, FBA1-PP7sl, GFA1-PP7sl and PGK1-PP7sl were generated by standard PCR based methods (Longtine et al., 1998). RPL28(Q38K) was generated by plating wild-type cells on 3 mg/mL cycloheximide plates, selecting for suppressors, backcrossing the suppressors at least three times and confirming the mutation by sequencing. HIS3 was generated by PCR replacement of the his3-11,15 allele using HIS3 from pRS303. leu2-3,112?::CG-LEU2::pGPD1-OsTIR1, his3-11,15?::CG-HIS3::pGPD1-OsTIR1, trp1-1?::CG-TRP1::pGPD1-LexA-EBD-B112, his3-11,15?::CG-HIS3::pGPD1-LexA-EBD-B112 and SCO2::p4xLexOcyc1-3xGST-V5-24xPP7sl-tCYC1-NatNT2 were generated by transforming strains with plasmids pKW2830 (PmeI digested), pKW2874 (PmeI digested), pKW3908 (SwaI digested), pKW4073 (SwaI digested) and pKW4190 (NotI/AscI digested) respectively. Czytaj dalej