Inside the vitro follicle incubation that have radiolabeled steroid precursors

Inside the vitro follicle incubation that have radiolabeled steroid precursors
Seafood and testing

Into the spawning year (later booleaf wrasse was in fact stuck from the hook up and you will range into the coastal oceans nearby the Fisheries Lookup Research, Kyushu College and transferred to brand new lab. Seafood have been stored in 500-litre fiberglass tanks that have filtered seawater, significantly less than pure day-size and you may h2o temperature, and fed krill and you may alive hermit crab once a day. Shortly after verifying each day spawning, cuatro–six lady seafood (fat – g, full duration 11step 3–159 mm) were tested at the , , , and you may time. Fish were anesthetized that have 2-phenoxyethanol (three hundred ppm), and you may bloodstream trials was in fact collected on the caudal ship using syringes suitable having 25-g getting 20 min. The broke up solution try held at ?30°C up until assayed for steroid peak. After bloodstream testing, fish have been slain of the decapitation, while the ovaries were dissected out. Having ovarian histology, short ovarian fragments was in fact fixed from inside the Bouin’s services, dried, and you may inserted into the Technovit resin (Kulzer, Wehrheim). The latest developmental values regarding oocytes was in fact in earlier times reported https://datingranking.net/tr/latinomeetup-inceleme/ (Matsuyama ainsi que al., 1998b).

The fresh new developmental values of one’s largest oocytes on the fish accumulated on , , and you can hour was in fact tertiary yolk (TY), early migratory nucleus (EMN), and later migratory nucleus (LMN) stages, respectively. The most significant follicles about fish sampled on time, where germinal vesicle breakdown (GVBD) got already taken place plus the cytoplasm was transparent due to yolk proteolysis and you may hydration, have been also known as adult (M) phase. Czytaj dalej